Extended diagnostic value of autologous serum skin test and basophil CD63 expression assay in chronic urticaria.
نویسندگان
چکیده
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منابع مشابه
Basophil Activation Test identifies the patients with Chronic Spontaneous Urticaria suffering the most active disease
INTRODUCTION The basophil activation test showing CD63 up regulation could be a specific and sensitive in vitro complementary text to the in vivo autologous serum skin test for the activity assessment of the patients suffering autoimmune chronic spontaneous urticaria. The aim of this study is to define the basophil activation test as a useful tool in clinical practice in order to identify those...
متن کاملSerum-induced basophil CD63 expression by means of a tricolour flow cytometric method for the in vitro diagnosis of chronic urticaria.
BACKGROUND Functional autoantibodies against the alpha-chain of the high-affinity IgE receptor (FcepsilonRIalpha) identify a subset of patients with chronic urticaria (CU) due to autoreactivity, as assessed by an in vivo positive response to autologous serum skin test (ASST). We performed a study to standardize the serum-induced basophil activation assay by flow cytometry (FCM) using a new tric...
متن کاملEvaluation of the utility of autologous serum skin test and the efficacy of autologous serum therapy in chronic spontaneous urticaria
Background: Autologous serum skin test (ASST) evaluates the presence of any serum histamine-releasing factors and histamine-releasing autoantibodies. Autologous serum therapy (AST) is a therapy in which repeated injections of autologous serum are administered intramuscularly for treatment of chronicspontaneous urticaria (CSU). The aim of this study is to evaluate the advantages and compare the ...
متن کاملPositive CD63 Basophil Activation Tests Are Common in Children with Chronic Spontaneous Urticaria and Linked to High Disease Activity.
BACKGROUND The basophil activation test (BAT) using CD63 expression is a sensitive and specific tool for the diagnostic workup of autoimmune chronic spontaneous urticaria (CSU). The definition of a positive BAT is directly dependent on the reference range and the cutoff values established in control populations. As of now, the pediatric reference range and cutoff values of the CD63 BAT remain t...
متن کاملBasophil Markers for Identification and Activation in the Indirect Basophil Activation Test by Flow Cytometry for Diagnosis of Autoimmune Urticaria
BACKGROUND The indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis. We aimed to identify better donor basophils (from atopic vs. non-atopic donors and interleukin-3 primed vs. unprimed basophils) and improve basophil identification and activation markers (eotaxin CC chemokine receptor-3 [CCR3] vs. CD123 and CD63 vs. CD203c). METHODS Do...
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ورودعنوان ژورنال:
- The British journal of dermatology
دوره 168 3 شماره
صفحات -
تاریخ انتشار 2013